The paper states, ``There is no evidence that GluD receptors act as ligand-gated ion channels.''Published in Proc Natl Acad Sci magazineit was done。
Delta receptors (GluD1 and GluD2)、A member of the ionotropic glutamate receptor family、plays a central role in many neurodevelopmental and psychiatric disorders。GluD is、Not related to ion channel activity、By forming a tripartite complex across synapses with the secreted synaptogenic molecules Cbln and neurexin (Nrxn).、Controls synapse formation and maturation。On the other hand、in recent years、Only when GluD2 forms the Nrxn/Cbln/GluD2 complex、It was reported that it functions as an ion channel that responds to D-serine and glycine.。Nozawa-kun, together with Hayashi-san、We、Currents induced by D-serine and glycine in ectopic cells and neurons、Proved that GluD is not directly involved as an ion channel。This finding is、It makes an important contribution to the ongoing discussion on the function of GluD.。In collaboration with Pierre Paoletti laboratory、Mr. Ito and Laura Piot acted as first authors.。

kainate-type glutamate receptor (KAR)A paper on the function of synapse formation is available online.became。KAR is、through ionotropic and metabotropic effects.、Involved in various neuropsychiatric and neurological disorders。However、Compared to AMPA-type and NMDA-type glutamate receptors,、The biological properties of KARs remain unclear in many ways。In this research、K.A.R.、Completely separate from ion channels and metabotropic effects、We revealed that it acts as a synapse organizer that creates synapses themselves.。In joint research with the Juan Lerma laboratory in Spain.、Associate Professor Kakegawa and Ana Paternain are first authors。For Press releaseClick here. 。

A paper co-authored with Jaime de Juan-Sanz of the Sorbonne University in France was published.Published in Cell Reportsit was done。LGI1, like Cbln1, is a synaptogenic molecule that belongs to the class of "extracellular scaffold proteins."。On this occasion、Like Cbln1, LGI1 is also secreted in response to neural activity and promotes synapse formation.、It was also found that it suppresses glutamate release.。Interestingly, Cbln1 is tetanus toxin (TeNT)(VAMP1-3 independent)、Secreted by Syntaxin-4 and SNAP49-dependent SNAREs。In contrast, LGI1 secretion was partially inhibited by TeNT.、Because it does not depend on SNAP29、found to be released by separate SNARE complexes。Yuzaki Lab provided analysis technology for the SNARE complex developed by Ibata。

The 163rd Brain Club welcomed Dr. Masanori Matsuzaki (University of Tokyo School of Medicine) on April 30, 2024。The title of the presentation is "Functional dynamics of intercortical circuits and cortical-subcortical circuits governing behavior and cognition."。Using mice and common marmosets、In the evolution from rodents to primates、We study how cerebral cortical circuits and their functional dynamics change to realize higher-order behavior and cognition.、We heard about how the activity of the cerebral cortex is being elucidated by optical measurement and optical manipulation while performing a task with the head fixed.。

 Experimental Medicine Special Issue April 2024 “Neuroscience opened up by large-scale data and AI”A review article by Mr. Takano and Mr. Sogabe titled ``The shape of synapses elucidated by proximity labeling and expansion microscopy'' was published in。

Mr. Takano、Promoted as an independent associate professor in the field of brain function and molecular systems at Kyushu University Institute of Advanced Research and Biodefense Medicine.。Thank you for meeting me! Busy with moving work、We had a small celebration party with champagne.。

2024Yuzaki at the Imperial Hotel on March 3, 2017.Purple Ribbon Medal Celebrationwas held。Despite the sunny Sunday、Approximately 100 people gathered from all over the country.。Thank you very much again。(Tokyo Marathon was being held on the road in front of the hotel.)。）

It was canceled for a while due to the coronavirus pandemic.Physiology class reunion was held。In the first half, there was a lecture by Professor Ayako Ishida, who studied abroad at the Yuzaki Lab and now works independently as a team leader at RIKEN CBS.、In the second half, we moved to the restaurant park to exchange opinions.、And it ended with the regular chorus of young blood.。

in the central nervous system、Astrocytes clear glutamate from the synaptic cleft.、Achieves proper synaptic function。However、How does the astrocytic glutamate transporter GLAST function around synapses?、it was still unclear。In this paper、Cell adhesion molecule (DSCAM) expressed in Purkinje cells、By controlling the localization of GLAST expressed in Bergmann glia、Climbing fibers were shown to be involved in Purkinje cell synapse formation and cerebellar motor learning.。This is a huge amount of work done by Mr. Dewa of Hoshino Lab.。For the Yuzaki lab, Kakegawa was in charge of electrophysiological analysis and eye movement learning test.。Nat Commun. 15:458, 2024.

How to covalently chemically label proteins without genetic manipulation、A powerful method for analyzing receptors。However、Selective target receptor labeling in the brain has not yet been established。there、In this research led by Mr. Nonaka of Kyoto University's Hamachi Laboratory,、using ligand-directed chemical reactions、We show that synthetic probes can selectively bind to target endogenous receptors in the brain of living mice。Kakegawa of Yuzaki Lab、As part of the joint research between CREST and ERATO, Arai、In this study, we showed that chemical labeling does not change receptor function.。Proc Natl Acad Sci USA. 121:e2313887121, 2024

GABA acts as a ligand for GluD1、A paper by Pierre Paoletti, Radu Aricescu et al. that reported that it promotes long-term potentiation of inhibitory synapses.（Science 2023)Mr. Ito wrote an explanatory article, Research Highlight, about (Ito and Yuzaki, Cell Research)。

Sponsored by Dept Biomedicine at the University of Bergen, NorwayAt BBB Seminar、Yuzaki gives a lectureThe we。It's a polar night and there's almost no daylight.、It was a rare heavy snow day even in Bergen, but we enjoyed a lively discussion.。